Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro
نویسندگان
چکیده
A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.
منابع مشابه
Quantitative studies of pinocytosis. II. Kinetics of protein uptake and digestion by rat yolk sac cultured in vitro
Pinocytic uptake of 125I-labeled bovine serum albumin by 17.5-day rat visceral yolk sac cultured in vitro has been examined. Uptake was followed by intracellular digestion and, after an initial period, the content of radioactivity in the tissue itself remained constant during the incubation. Radiolabel was returned to the culture medium predominantly as (125I)iodotyrosine; exocytosis of undiges...
متن کاملEthanol-induced inhibition of pinocytosis and proteolysis in rat yolk sac in vitro.
Pinocytic capture of 125I-labelled polyvinylpyrrolidone and of formaldehyde-denatured 125I-labelled bovine serum albumin by 17.5-day rat visceral yolk sacs incubated in vitro was rapidly and strongly inhibited by low concentrations (0.01 and 0.05%, v/v) of ethanol. The induced inhibition of pinocytosis was readily reversible, but a marked lag was observed before ethanol-exposed tissue regained ...
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The highly pinocytic epithelial cells of the visceral yolk sac from 17.5-day rat conceptuses were used as a model in which to induce engorgement of the vacuolar system by direct accumulation of substances that are not hydrolysed by lysosomal enzymes. The ultra-structural appearances of these cells in pregnant animals that 24-48h before had received intraperitoneal injections of Triton WR-1339, ...
متن کاملQUANTITATIVE STUDIES OF PINOCYTOSIS I. Kinetics of Uptake of [~sI]Polyvinylpyrrolidone by Rat Yolk Sac Cultured In Vitro
A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of ~25I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentrat ion range 0 .15-24 ~tg/ml, the a2~I-labeled polyvinylpyrrolidone neither stimulated nor inhibi...
متن کاملEthanol-induced inhibition of pinocytosis and proteoiysis in rat yolk sac in vitro
Pinocytic capture of I-labelled polyvinylpyrrolidone and of formaldehyde-denatured ^I-labelled bovine serum albumin by 17-5-day rat visceral yolk sacs incubated in vitro was rapidly and strongly inhibited by low concentrations (001 and 005%, v/v) of ethanol. The induced inhibition of pinocytosis was readily reversible, but a marked lag was observed before ethanol-exposed tissue regained its ful...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 64 شماره
صفحات -
تاریخ انتشار 1975